evaluation of pdx-1 gene expression in insulin producing cells, derived from embryonal carcinoma stem cells

background and aims: understanding β-cell function at the molecular level will likely facilitate the development of β-cells manufacture techniques. the aim of the present study was to investigate the pancreatic and duodenal homeobox 1 (pdx-1) gene expression into dtz-stained cellular clusters originating from emberyonal carcinoma cells. methods: this implemental -fundamental study was accomplished on differentiation of stem cells into insulin producing cells (ipcs). the conditioned medium of cultured pancreas from one-week newborn mouse was used for differentiation of p19. ebs formed by a 24h suspension culture of p19 ec cells. in order to induce the difrentiation,, different concentrations of conditioned medium (25%, 50%, 75% and 100%) were added to culture medium. dithizone staininig was used for detecting the differentiated cells derived from ebs in vitro. insulin-proinsulin production and insulin receptor beta were determined by immunofluorescence. the expression of pdx-1 gene was also analyzed by reverse transcriptase-polymerase chain reaction. data were analyzed using one way anova and dunkana test. results: differentiated cell clusters appeared after approximately 7 days induction. the peak response of differentiation was at the concentration of 50% conditioned medium. expression of pdx-1 gene was observed in the differentiated cell clusters. expression of insulin-proinsulin and insulin receptor beta markers in the differentiated cells was confirmed by immunofluorescence. conclusion: the pancreas conditioned medium could influence p19 cells differentiation into insulin producing cells. so findings of this study could facilitate producing the β-cells